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Life Science Research and Sustainable Development ISBN: 978-98-84663-33-9

Material and Methods:
The fluoride waters obtained from nearby areas and were tested in public health Lab. The report
indicates that, the bore water (F) contained 4.285mg/lit. Cyprinus carpio, ranging between
weighing about 250gm.were collected from a nursery pond at Sawargaon in Umri Tahsil of
Nanded district. The animals were brought to the laboratory and were acclimatized to lab
condition for four days where they fade with rice and groundnut cake. Fish was exposed in lethal
& sub lethal con. of fluoride at different time interval. One group served as a control, LC50 was
recorded at 96h.
Collection of Blood: - The blood was collected by direct heart puncturing using sterile disposable
plastic syringe with a 22-gauge needle (Molnar, 1960). The blood sample was taken in a tube
rinsed with EDTA as an anticoagulant, & was mixed gently by rotation and stored in a
refrigerator at 4 C. Preserved blood sample was used for hematological studies. Hematological
o
parameters were determined by fully automated Cell Counter (Trivitron Selenium Jr.) &
compared with control.
Results: In the present study, in control, WBC count was highest in 72h & 48h followed by 24h &
96h. The HB was highest in 72h, followed by 24h & in 48h. The lowest was in 96h. The RBC count
was highest in 72h followed by 48h 96h & in 24h. The hematocrit HCT percentage were highest
in the 72h followed by 48h 24h & lowest was in 96h. The MCV was highest in 24h followed by
48h & 72h. Lowest was in 96h. The MCH in control was highest in 24h followed by 96h, 72h,
lowest was in 48h. MCHC was highest in 24h followed by 72h and 96h & lowest were in 48h. as
represented in graph (A) when, fish, Cyprinus carpio was exposed in lethal as well as sub lethal
concentrations of fluoride, significant changes were observed on the haematological parameters.
WBCs was significantly (P>0.05, P<0.05) decreased in the 24h, 48h & 72h. In 96h it was highly
insignificantly decreased. In 24h, 48 &72h it was significantly (P<0.05) decreased. In 96h WBC
was insignificantly decreased. The HB was increased insignificantly in 96h. The insignificantly
decreased HB was in 24h & 48h. While significantly (P>0.05) decreased in 72h. The RBCs was
decreased in all the duration of exposure. Significant (P<0.05) decreased was observed in 48h. In
the 24h RBCs was decreased significantly (P>0.05). In 72h decreased insignificantly. In 96h it was
insignificant decreased. The HCT, decreased in all duration & significantly (P>0.05) decreased in
the 48h & 72h. In 48h, it was decreased insignificantly. The MCV was decreased all the duration.
MCV was in 24h and 72h, was found decreased significantly (P>0.05). In 48h & 96h was decreased
insignificantly. The MCH was highly elevated significantly during 48h. In 24h it was decreased
significantly (P>0.05). In 72h & 96h decreased insignificantly. The MCHC increased in all
duration. & highly elevated significantly (P>0.05) in the 48h, & in 72h increased significantly
(P>0.05). In 96h increased insignificantly. In 24h, it was found significant equal (P=0.05). As
represented in graph (B).
In Sub lethal, WBCs was significantly decreased in all the duration. In 96h it was insignificantly
decreased. In 24h it was significantly decreased (P=0.05).In 48h &72h it was significantly (P>0.05)
decreased. In 96h, it was insignificantly decreased. The HB was increased insignificantly in
96h.while insignificantly decreased was in 24h, 48h. It was significantly (P>0.01) decreased in72h.
The RBC was increased insignificantly in 48h & in 24h it was decreased insignificantly. In 72h, it
was decreased significantly (P>0.05). In24h & 96h, it was insignificantly decreased. The HCT was
increased insignificantly. In the 48h & 72h, it was significantly (P>0.05) decreased. The MCV was
increased insignificantly in 96h. MCV in 24h & 48h significantly (P>0.05) decreased. In 48h, &
96h, it was decreased insignificantly. The MCH was observed elevated in 48h & decreased
insignificantly. In 24h it was decreased insignificantly. Followed by 72h it was decreased

https://jesjalna.org/Zoology-Publications/index.html 68 Department of Zoology, J. E. S. College, Jalna