Page 137 - RUT Publisher

Life Science Research and Sustainable Development ISBN: 978-98-84663-33-9

REVIEW ARTICLE

Fungal Laccase: Applications, Screening and Optimization of cultural condition for laccase
production.

2
1 V. R. Mhaske and M. S. Wadikar
1 Department of Botany Shikshan Maharshi Dnyandeo Mohekar Mahavidyalaya, Kalamb. Dist:
Osmanabad. (MS) 413507.

2 Department of Botany R. B. Attal Arts, Science and Commerce College, Georai. Dist: Beed. (MS)

431127.

Keywords: Abstract:
Fungal In the recent years, enzymes have gained great importance in
Laccase: industries. Laccases are one of them which are widely present in
Applications, nature. Laccases are found in plants, insects and bacteria, but the most
Screening and important sources of these enzymes are fungi. Most of the laccases
Optimization studied are of fungal origin especially from the white-rot fungi class.
Fungal laccases play an important role in plant pathogenesis, pigment
production and degradation of lignocellulosic materials. Application
of laccase includes Textile dye decolourization, Bioremediation, Food
industry, Pulp and paper industry, Pharmaceutical industry and
Cosmetic Industry. Laccase production may be affected by
fermentation factors such as, medium composition, nutrient sources,
pH and temperature. This paper reviews the application, function,
screening and optimization of cultural condition for laccase
production.

1.Intoduction:
Laccase:
Laccases are the oldest and most studied enzyamatic systems (benzenediol:oxygen
oxidoredutases, EC 1.10.3.2). Yoshida in 1883 first described laccase from the exudates of the
Japanese lacquer tree, Rhus vernicifera and in 1896 laccase was demonstrated to be present in fungi
for the first time by Bertrand and Laborde (Thurston, 1994).
Laccase is an enzyme that has potential ability of oxidation. These enzyme catalyzes one
electron oxidation of wide variety of organic and inorganic substrates including mono-, di- and
polyphenols, aminophenols, methoxyphenols, aromatic amines and ascorbate with concomitant
four electron reduction of oxygen to water (Vernekar and Lele, 2009). There are several
compounds that have been used as substrates for laccase viz. 2,2'-azinobis-(3-ethylbenzthiazoline-
6-sulfonate) (ABTS), tannic acid, syringaldazine, o-dianisidine, guaiacol, dimethoxyphenol and
naphthol.
2. Sources of laccase:
There are diverse sources of laccase producing organism like fungi, plants, bacteria and
insects. Laccases were reported from plants include Lacquer, Mango, Mung bean, Peach, Pine,

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