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Life Science Research and Sustainable Development                                   ISBN: 978-98-84663-33-9

               shortening of the body, elongation of the body, oozing out of coelomic fluid, preclitellar swelling,
               body constriction and segmental bulging Sivakumar (2015). In general, earthworms are effective
               accumulators of metals from the soils leading to compartmentalization, storage, or excretion of
               metal ions from the most sensitive tissues.
                       They  apparently  have  well  developed,  specific  trafficking  and  storage  pathways  and
               redistribution capacity to regulate heavy metals, especially essential trace metals, in their bodies
               that may lead to balance between uptake and excretion. The regulatory capacity of metals can
               partly explain the ability of some earthworm species to live even in highly metal contaminated
               areas. Moreover, metal body did not increase with increasing soil metal concentrations, not even
               with these timated bioavailable fractions of the metals. Thus, the metal regulation may also have
               contributed to the development of metal resistance observed in some earthworm populations
               Lukkari et al., (2004). Harmfulness of certain metal concentrations measured from the earthworms
               may be difficult to evaluate. Especially in the field, body burdens affecting survival, growth, and
               reproduction  of  earthworms  seem  to  be  site  and  species-specific.  In  addition,  responses  of
               earthworms to metal contamination are modified by several other environmental factors Latha &
               Mahaboob (2016).
               Conclusion
                       The  conclusion  of  a  study  on  the  impact  of  heavy  metals  (mercuric  chloride,  copper
               sulphate,  and zinc  sulphate)  on  the  survivability  of  earthworms would  summarize  the  main
               findings of the research, discussing how exposure to these heavy metals affected the survival of
               the earthworms. It would also highlight any notable trends or patterns that emerged from the
               study, such as whether one heavy metal had a more pronounced effect on earthworm survival
               than the others, or whether there was any dose-response relationships observed. Additionally,
               the conclusion would relate the results to other existing studies on the topic, and discuss the
               ecological and environmental implications of the findings.
                       It could be like: "The results of this study demonstrate that exposure to mercuric chloride,
               copper sulphate, and zinc sulphate heavy metals have negative effects on the survival rate of
               earthworms.  The  study reveals  that  mercuric chloride  has  the highest  toxic  effect  among  the
               heavy metals used in this study. The findings of this research have ecological and environmental
               implications as earthworms play an important role in ecosystem functioning. Further research
               should be conducted to better understand the mechanisms of heavy metal toxicity in earthworms,
               as well as to evaluate the potential long-term impacts of heavy metal pollution on earthworm
               populations."
               References:
                   1.  Abbott, W.S. (1925) A Method of Computing the Effectiveness of an Insecticide. Journal
                       of Economic Entomology, 18, 265-267.
                   2.  APHA (1989) Standard Methods for the Examination of Water and Wastewater, Part 3,
                       Determination of Metals. 17th, American Public Health Association, Washington DC, 164.
                   3.  Dash M C and Patra UC 1977Density, biomass and energy budget of a tropical earthworm
                       population from a grassland site in Orissa, India; Rev. Ecol. Bio. Sol. 14461-471
                   4.  Finney, D.J. (1971) Probit Analysis. 3rd Edition, Cambridge University Press, Cambridge.
                   5.  Kapil Parihar1, Rajeev Kumar2, Mahipal Singh Sankhla1 Shefali3 (2019): Impact of Heavy
                       Metals on Survivability of Earthworms international medico-legal reporter journal, vol, 2,
                       issue-3, November 2019.
                   6.  Latha, V., &MahaboobBasha, P. (2016). Extent of Heavy Metal Accumulation in Sewage
                       Irrigated Soils and Their Impact on Distribution of Earthworm Communities: Linking


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