Page 143 - RUT Publisher

Life Science Research and Sustainable Development ISBN: 978-98-84663-33-9

laccase. Most of the studies show that pH between 4.5 to 6.0 is suitable for laccase production.
The optimal temperature of laccase differs from one strain to another. It has been found that 25
0
to 30 is the optimal temperature for laccase production.
0
Banerjee and Vohra (1991) optimized proper culture conditions for Curvularia sp. and
concluded that the initial pH 4.0 and temperature 30 C were found to be significant for maximum
0
laccase production from Curvularia sp. Arora and Gill (2005) carried out optimization of proper
culture conditions for laccase production from white rot fungus Phlebia floridensis and reported
that, laccase production and manganese peroxidase production was found to be maximum on
pH 4.5. Janusz et al., (2006) concluded that the soil dwelling fungus Rhizoctonia praticola represents
a new source of extracellular laccase and for the highest production of laccase enzyme, fungus
requires a high value of initial medium pH (7.5-8).
Niladevi and Prema (2008) studied optimization of various cultural and nutritional
parameters for the production of laccase by Streptomyces psammoticus and showed that the
maximum laccase yield was attained at pH 7.5 and temperature 32 C. Rajeswari and Parvatham
0
(2011) standardized optimum cultural conditions for laccase production from Aspergillus sp. and
reported that pH 4.6 and temperature 40°C were found to be optimum and computed half-life of
laccase activity in minutes were 120 (40°C), 60 (50°C) and 35 (60°C) respectively. Christie and
Shanmugam (2012) carried out optimization of Alternaria arborescence and Fusarium oxysporium
for laccase enzyme production and showed maximum production of enzyme in Alternaria
arborescence (800U/l) at 30 C and 4.5 pH followed by Fusarium oxysporium with (600 U/l) at 45 C
0
0
and 5 pH after 15 days of incubation.
Manimozhi and Kaviyarasan (2012) isolated Agaricus heterocystis and carried out an
experiment on optimum temperature and pH for fungal mycelial biomass and laccase activity
and reported that the highest yield of biomass was at 30 C and pH 6.5 whereas laccase activity
0
was found to be maximum at 25 C and pH 5.5. Fonseca et al., (2013) studied influence of culture
0
conditions on laccase production, growth and isoenzymes patterns in native white rot fungi and
concluded that the selected four strains showed attractive and alternative source for laccase
production influenced by combined effects of temperature and pH. Potential strain shows highest
laccase activity at 29 C and pH 4.5.
0
Rathinasamy and Thayumanavan (2014) observed that the growth of the fungus Pleurotus
ostreatus IMI 395545 and the production of laccase were highly influenced by effective controlling.
The factors like pH, temperature and percentage of dissolved oxygen tension (DOT) provision
available in the bioreactor. Vantamuri et al., (2015) isolated different fungal isolates and screened
them for laccase production with guaiacol as an indicator compound. Out of 9 isolates tested only
2 isolates have the ability to produce laccase. Potent fungus was morphologically identified as
Coprinus comatus. The laccase activity was highest with sucrose as carbon source and yeast extract
as nitrogen source. The highest production of laccase was found to be at pH 5 and the optimum
temperature for production was recorded at 30 C.
0
Sidhu et al., (2017) reported the optimum pH, incubation temperature and incubation
period for the laccase production in submerged culture were found to be pH 6, temperature 30 C,
0
7 day incubation period respectively.
7. References:
Abdel-Azeem, A.M. and Salem, F. M. (2012). Biodiversity of laccase producing fungi in Egypt.
Mycosphere 3(5): 900–920.

https://jesjalna.org/Zoology-Publications/index.html 135 Department of Zoology, J. E. S. College, Jalna