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Life Science Research and Sustainable Development ISBN: 978-98-84663-33-9

Morphological details of the bacterial samples are presented in Table 02. Morphologically
11 bacterial strains viz. RH 01, RH 02, RH 03, RH 04, RH 06, RH 08, RH 09, 09, RH 11, RH 13, RH
14 and RH 15 were identical showing similar morphological characters. These strains are
grouped as Group-I. While 05 bacterial strains RH 05, RH 07, RH 10, RH 12 and RH 16 were
different from one another and they are grouped in Group-II. Bacterial cells of Group-I were
gram negative. While bacterial cell of Group-II were Gram positive in staining. The cell size of
Group-I varies from 1.45 µm to 1.88 µm. Group-II cell size was larger than that of Group-I which
was varying between 2.40 µm to 2.68 µm. All the bacterial strains of Group-I was rod shaped but
in Group-II, RH 05, RH 07, RH 10, RH 12 was large rod shaped while bacterial cell of RH 16 was
coccus in shape.
All the bacterial colonies of Group-I strains were creamy white colored on the special
media. And in Group-II, Strain RH 05, RH 07, RH 16 showed creamy pale orange color while RH
10 and RH 12 showed yellow color while. The bacterial colonies of Group-I strains were circular
in shape while Group-II showed irregular shape. Colony size of the Group-I was ranging between
1.25 mm to 1.39 mm while colony size of Group-II was ranging between 1.44 mm to 1.58 mm.
Appearance of the Group-I bacterial strain is glistering while Group-II showed opaque colonies.
The bacterial strain margins of Group-I showed smooth margins while Group-II showed rough
and smooth margin. These morphological characters resembled A. diazotrophicus.
Similar bacterial cell and colony morphology of A. diazotrophicus was described by various
research workers (Fuentes-Ramirez et al., 1998; Madhaiyan et al., 2004; Chawla et al., 2014).
Fuentes-Ramirez et al., (1998) reported colonization of sugarcane by A. diazotrophicus inhibited by
high N-fertilization. Reis et al., (1994) suggested improved methodology for isolation of A.
diazotrophicus and confirmation of its endophytic habitat. Reis et al., in 2015 described role of
nitrogen fixing family Acetobacteraceae in agriculture. Similar characterization of
Gluconacetobacter diazotrophicus is reported by Ahmed et al., (2016) isolated from sugarcane
cultivated in Upper Egypt. Kuchekar and Pawar (2019) also studied morphological
characterization of Azotobacter spp. from various localities of Aurangabad district (MS). James et
al., (1994) reported presence of A. diazotrophicus as nitrogen-fixing bacterium in sugarcane.

REFERENCES
Adeleke, B.S and O.O. Babalola (2021) The endosphere microbial communities, a great promise
in agriculture. Journal of Plant Growth Regulation, (2): 2-19.
Ahmed, H.F. F.H.Badawy; S.M. Mahmoud and M.M. El-Dosouky (2016) Characterization of
Gluconacetobacter diazotrophicus Isolated from Sugarcane (Saccharum officinarum) Cultivated in
Upper Egypt. Assiut Journal Agriculture Science, 47(6-2): 569-582.
Chawla, N., M. Phour, S. Suneja, S. Sangwaan and Sneh Goyal (2014) Gluconacetobacter
diazotrophicus: An overview. Research in Environment and Life Sciences, 7(1): 1-10
Cheng, Qi (2008) Perspectives in biological nitrogen fixation research. Journal of Integrative Plant
Biology, 50(7):784-796
Cholke, S.P. (2015) Assessment of ground water quality in Shrirampur Tehsil: A GIS- Integrated
Approach. International Journal of Science and Research (IJSR), 5(5): 436-439
Dong, Z., McCully, M.E. and Canny, M.J. (1997) Does Acetobacter diazotrophicus live and move
in the xylem of sugarcane stems.Anatomical and physiological data. Ann. Bot. 80(2):147-158.
Fuentes-Ramirez, L.E., T. Jimenez-Salgado, I.R. Abarco-ocampo and J. Cambblero-Mellado (1993)
Acetobacter diazotrophicus, an indoleacetic acid producing bacterium isolated from sugarcane
cultivars of Mexico. Plant and Soil, 154: 145-150.

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